HILPADA Knockout cell line (HCT116)

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HILPADA Knockout cell line (HCT116) Order

HILPADA Knockout cell line (HCT116)
Catalog#
YKO-KP270
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
HILPADA Knockout cell line (HCT116)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
HILPADA Knockout cell line (HCT116)
Species
Human
Gene Name
HILPADA
Gene ID
29923
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the HILPADA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, HILPADA gene knockout HILPADA Knockout cell line (HCT116) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
HILPADA Knockout cell line (HCT116)
HILPADA Knockout cell line (HCT116)
Catalog#
YKO-KP270
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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